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Biofuel-Producing Lactobacillus Strain

Great Lakes Bioenergy Research Center

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Technology Marketing Summary

Microbial transformation of biomass into biofuels remains an important part of the United States’ strategy to reduce its dependency on fossil fuels. To produce ethanol from biomass, microbes must be able to efficiently metabolize plant sugars into ethanol under industrial fermentation stresses.

Naturally occurring microorganisms have not evolved to thrive in such industrial processes. Researchers have approached this problem by engineering strains that leverage the genetic strengths of select organisms to supplement the deficiencies in others.

So far, engineered strains have failed to maximize yields under industrial conditions. Continued strain discovery and optimization remains a vibrant research area.


A UW–Madison researcher and others have modified a Lactobacillus casei strain that exhibits the highest ethanol conversion rates yet reported from the genus.

L. casei naturally combines many characteristics of an ideal strain when compared to microorganisms typically considered for biofuel production, like Saccharomyces cerevisiae,Zymomonas mobilisEscherichia coli and Clostridium sp., which all suffer from various deficiencies. A L. casei strain exhibiting high conversion rates could represent a novel, more efficient path to market for ethanol production.

The modified bacterium is derived from L. casei strain 12A. It is made by (i) inactivating genes that encode a competing lactate enzyme and (ii) introducing genes from another organism (Zymomonas mobilis) that encode a pyruvate decarboxylase and an alcohol dehydrogenase II.

  • Outperforms all other lactobacilli
  • L. casei 12A is an ideal candidate
Applications and Industries
  • Biofuel (ethanol) production
  • Potential adaption to higher margin biochemical products and second generation biofuels such as isobutanol
More Information

Related Technologies: 

WARF reference number P100228US02 describes a recombinant yeast strain with improved ethanol tolerance.

WARF reference number P130153US01 describes modified yeast that show improved xylose fermentation and toxin tolerance.

Technology Status
Development StageAvailabilityPublishedLast Updated

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To: Mark Staudt<>