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Agrobacterium-mediated transformation of lipomyces

United States Patent

9,914,932
March 13, 2018
View the Complete Patent at the US Patent & Trademark Office
Pacific Northwest National Laboratory - Visit the Technology Commercialization Program Website
This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.
Dai; Ziyu (Richland, WA), Magnuson; Jon K. (Richland, WA), Deng; Shuang (Richland, WA), Bruno; Kenneth S. (Richland, WA), Culley; David E. (Richland, WA)
Battelle Memorial Institute (Richland, WA)
14/ 540,818
20160138031
November 13, 2014
ACKNOWLEDGMENT OF GOVERNMENT SUPPORT This invention was made with government support under Contract DE-AC05-76RL01830 awarded by the U.S. Department of Energy. The government has certain rights in the invention.