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Engineered microbes and methods for microbial oil production

United States Patent

January 9, 2018
View the Complete Patent at the US Patent & Trademark Office
Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.
Stephanopoulos; Gregory (Winchester, MA), Tai; Mitchell (Seattle, WA), Chakraborty; Sagar (Cambridge, MA)
Massachusetts Institute of Technology (Cambridge, MA)
14/ 547,304
November 19, 2014
GOVERNMENT SUPPORT This invention was made with government support under Grant No. DE-AR0000059 awarded by the US Department of Energy. The government has certain rights in the invention.