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Process and genes for expression and overexpression of active [FeFe] hydrogenases

United States Patent

September 16, 2014
View the Complete Patent at the US Patent & Trademark Office
National Renewable Energy Laboratory - Visit the NREL Technology Transfer Website
A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.
Seibert; Michael (Lakewood, CO), King; Paul W. (Golden, CO), Ghirardi; Maria Lucia (Lakewood, CO), Posewitz; Matthew C. (Golden, CO), Smolinski; Sharon L. (Littleton, CO)
Alliance for Sustainable Energy, LLC (Golden, CO)
11/ 573,035
February 28, 2006
CONTRACTUAL ORIGIN OF THE INVENTION The United States Government has rights in this invention pursuant to Contract No. DE-AC36-99GO10337 between the United States Department of Energy and the Midwest Research Institute.