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Oxygen-resistant hydrogenases and methods for designing and making same

United States Patent

March 4, 2014
View the Complete Patent at the US Patent & Trademark Office
National Renewable Energy Laboratory - Visit the NREL Technology Transfer Website
The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H.sub.2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing the transformed, oxygen insensitive, host cells in the bulk production of H.sub.2 in a light catalyzed reaction having water as the reactant.
King; Paul (Golden, CO), Ghirardi; Maria Lucia (Golden, CO), Seibert; Michael (Golden, CO)
Alliance for Sustainable Energy, LLC (Golden, CO)
12/ 360,756
January 27, 2009
GOVERNMENT INTEREST The United States Government has rights in this invention pursuant to contract DE-AC36-08GO28308 between the United States Department of Energy and the Alliance for Sustainable Energy, LLC, manager and operator of the National Renewable Energy Laboratory.