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Compositions and methods for the expression of selenoproteins in eukaryotic cells

United States Patent

September 25, 2012
View the Complete Patent at the US Patent & Trademark Office
Recombinant nucleic acid constructs for the efficient expression of eukaryotic selenoproteins and related methods for production of recombinant selenoproteins are provided. The nucleic acid constructs comprise novel selenocysteine insertion sequence (SECIS) elements. Certain novel SECIS elements of the invention contain non-canonical quartet sequences. Other novel SECIS elements provided by the invention are chimeric SECIS elements comprising a canonical SECIS element that contains a non-canonical quartet sequence and chimeric SECIS elements comprising a non-canonical SECIS element that contains a canonical quartet sequence. The novel SECIS elements of the invention facilitate the insertion of selenocysteine residues into recombinant polypeptides.
Gladyshev; Vadim (Lincoln, NE), Novoselov; Sergey (Puschino, RU)
Board of Regents of the University of Nebraska (Lincoln, NE)
12/ 428,007
April 22, 2009
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT This invention was made with government support under GM061603 awarded by the National Institutes of Health and with government support under DE-FG07-02ID14380 awarded by the Department of Energy. The government has certain rights in this invention.