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Genetically encoded fluorescent coumarin amino acids

United States Patent

June 5, 2012
View the Complete Patent at the US Patent & Trademark Office
The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the coumarin unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid L-(7-hydroxycoumarin-4-yl)ethylglycine and related translation systems.
Wang; Jiangyun (San Diego, CA), Xie; Jianming (San Diego, CA), Schultz; Peter G. (La Jolla, CA)
The Scripps Research Institute (La Jolla, CA)
12/ 227,383
May 21, 2007
STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH AND DEVELOPMENT This invention was made with government support from the Department of Energy under Grant No. ER46051. The government has certain rights to this invention.