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Flow cytometric detection method for DNA samples

United States Patent

July 5, 2011
View the Complete Patent at the US Patent & Trademark Office
Lawrence Livermore National Laboratory - Visit the Industrial Partnerships Office Website
Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM.TM. on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA.TM., on the 5' end.
Nasarabadi; Shanavaz (Livermore, CA), Langlois; Richard G. (Livermore, CA), Venkateswaran; Kodumudi S. (Round Rock, TX)
Lawrence Livermore National Security, LLC (Livermore, CA)
11/ 454,478
June 16, 2006
The United States Government has rights in this invention pursuant to Contract No. W-7405-ENG-48 between the United States Department of Energy and-the University of California for the operation of Lawrence Livermore National Laboratory.