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Use of CYP52A2A promoter to increase gene expression in yeast

United States Patent

January 6, 2004
View the Complete Patent at the US Patent & Trademark Office
A nucleic acid sequence including a CYP promoter operably linked to nucleic acid encoding a heterologous protein is provided to increase transcription of the nucleic acid. Expression vectors and host cells containing the nucleic acid sequence are also provided. The methods and compositions described herein are especially useful in the production of polycarboxylic acids by yeast cells.
Craft; David L. (Ft. Thomas, KY), Wilson; C. Ron (Loveland, OH), Eirich; Dudley (Milford, OH), Zhang; Yeyan (Mason, OH)
Cognis Corporation (Gulph Mills, PA)
09/ 911,781
July 24, 2001
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT This invention was funded, at least in part, under grants from the Department of Commerce, NIST-ATP Cooperative Agreement Number 70NANB8H4033 and the Department of Energy No. DE-FC36-95GO10099. The Government may therefore have certain rights in the invention.