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Polymer mixtures for high performance/high temperature separation in capillary electrophoresis, especially for long read DNA sequencing

United States Patent

November 5, 2002
View the Complete Patent at the US Patent & Trademark Office
A method of separating a mixture of biopolymers of various lengths using a blend of two linear hydrophilic polymers, particularly polyacrylamides (LPA) or substituted polyacrylamides, one with high weight-average molecular mass and another with low weight-average molecular mass, each present at a low concentration but wherein the concentration of the high weight-average molecular mass LPA is above its entanglement threshold, is disclosed. The matrix is excellent for use in methods of nucleic acid analysis, particularly long read length DNA sequencing.
Karger; Barry L. (Newton, MA), Kotler; Lev (Brighton, MA), Goetzinger; Wolfgang (Boston, MA), Zhou; Haihong (Boston, MA), Sosic; Zoran (Cambridge, MA), Salas-Solano; Oscar (San Francisco, CA)
Northeastern University (Boston, MA)
09/ 507,496
February 18, 2000
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT Part of the work leading to this invention was carried out with United States Government support provided under a grant from the Department of Energy, Grant No. DE-FG02-90ER-60985. Therefore, the U.S. Government has certain rights in this invention.