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Rapid method to detect duplex formation in sequencing by hybridization methods, a method for constructing containment structures for reagent interaction

United States Patent

October 15, 2002
View the Complete Patent at the US Patent & Trademark Office
Argonne National Laboratory - Visit the Technology Development and Commercialization Website
A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to polymerize into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units.
Mirzabekov; Andrei Darievich (Moscow, RU), Yershov; Gennadiy Moiseyevich (Zelenograd, RU), Guschin; Dmitry Yuryevich (Hinsale, IL), Gemmell; Margaret Anne (Western Springs, IL), Shick; Valentine V. (Moscow, RU), Proudnikov; Dmitri Y. (Samara, RU), Timofeev; Edward N. (Moscow, RU)
University of Chicago (Chicago, IL)
09/ 127,313
July 31, 1998
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT The United States Government has rights in this invention pursuant to Contract No. W-31-109-ENG-38 between the U.S. Department of Energy and the University of Chicago representing Argonne National Laboratory.