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DNA polymorphism identity determination using flow cytometry

United States Patent

September 11, 2001
View the Complete Patent at the US Patent & Trademark Office
Los Alamos National Laboratory - Visit the Technology Transfer Division Website
DNA polymorphism identity determination using flow cytometry. Primers designed to be immobilized on microspheres are allowed to anneal to the DNA strand under investigation, and are extended by either DNA polymerase using fluorescent dideoxynucleotides or ligated by DNA ligase to fluorescent reporter oligonucleotides. The fluorescence of either the dideoxynucleotide or the reporter oligonucleotide attached to the immobilized primer is measured by flow cytometry, thereby identifying the nucleotide polymorphism on the DNA strand.
Nolan; John P. (Santa Fe, NM), White; P. Scott (Los Alamos, NM), Cai; Hong (Los Alamos, NM)
The Regents of the University of California (Los Alamos, NM)
09/ 182,869
October 28, 1998
The present invention relates generally to the use of flow cytometry for the determination of DNA nucleotide base composition and, more particularly, to the use flow cytometry to determine the base identification of single nucleotide polymorphisms, including nucleotide polymorphisms, insertions, and deletions. This invention was made with government support under Contract No. W-7405-ENG-36 awarded by the US Department of Energy to The Regents of The University of California. The government has certain rights in this invention.