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Recombinant pinoresinol/lariciresinol reductase, recombinant dirigent protein, and methods of use

United States Patent

April 3, 2001
View the Complete Patent at the US Patent & Trademark Office
Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.
Lewis; Norman G. (Pullman, WA), Davin; Laurence B. (Pullman, WA), Dinkova-Kostova; Albena T. (Baltimore, MD), Fujita; Masayuki (Kagawa, JP), Gang; David R. (Ann Arbor, MI), Sarkanen; Simo (S. Minneapolis, MN), Ford; Joshua D. (Pullman, WA)
Washington State University Research Foundation (Pullman, Washington), Regents of the University of Minnesota (Minneapolis, MN)
09/ 475,316
December 30, 1999
GOVERNMENT RIGHTS This invention was funded in part by grant number DE-FG03-97ER20259 from the United States Department of Energy, by grant number MCB09631980 from the National Science Foundation, by grant number NAG100164 from the National Aeronautics and Space Administration, and by grant number 96-35103-3358 from the United States Department of Agriculture. The government has certain rights in this invention.