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Plant nitrogen regulatory P-PII genes

United States Patent

January 23, 2001
View the Complete Patent at the US Patent & Trademark Office
The present invention generally relates to plant nitrogen regulatory PII gene (hereinafter P-PII gene), a gene involved in regulating plant nitrogen metabolism. The invention provides P-PII nucleotide sequences, expression constructs comprising said nucleotide sequences, and host cells and plants having said constructs and, optionally expressing the P-PII gene from said constructs. The invention also provides substantially pure P-PII proteins. The P-PII nucleotide sequences and constructs of the inventor may be used to engineer organisms to overexpress wild-type or mutant P-PII regulatory protein. Engineered plants that overexpress or underexpress P-PII regulatory protein may have increased nitrogen assimilation capacity. Ergineered organisms may be used to produce P-PII proteins which, in turn, can be used for a variety of purposes including in vitro screening of herbicides. P-PII nucleotide sequences have additional uses as probes for isolating additional genomic clones having the promoters of P-PII gene. P-PII promoters are light- and/or sucrose-inducible and may be advantageously used in genetic engineering of plants.
Coruzzi; Gloria M. (New York, NY), Lam; Hon-Ming (Hong Kong, HK), Hsieh; Ming-Hsiun (Woodside, NY)
New York University (New York, NY)
08/ 899,330
July 23, 1997
This invention was made with U.S. government support under National Institute of Health grant number GM32877 and Department of Energy grant number DE-FG02-94ER20133 and National Science Foundation grant No. DIR-8908095. The U.S. government has certain rights in the invention.