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Polynucleotide constructs and methods of expression in a host cell

United States Patent Application

View the Complete Application at the US Patent & Trademark Office
Pacific Northwest National Laboratory - Visit the Technology Commercialization Program Website
The invention includes polynucleotide constructs that contain a polynucleotide promoter sequence from the phosphoglycerate kinase 1 (pgk1) gene from Rhizopus oryzae. The invention also includes methods of expressing a polypeptide in a host cell using a polynucleotide construct containing a 3-phosphoglycerate kinase (pgk) gene promoter sequence operably linked to a polynucleotide encoding a polypeptide of interest. The invention also includes a system for producing a polypeptide. The system has a first polynucleotide sequence containing a 3-phosphoglycerate kinase gene promoter sequence isolated from a fungal species, and a second polynucleotide sequence which has been isolated from a second species and which encodes a polypeptide. A host cell contains the first polynucleotide sequence operably linked to the second polynucleotide sequence. The host cell is from a different species than the fungal species from which the first polynucleotide sequence is isolated.
Gao, Johnway (Richland, WA), Anderson, Daniel B. (Pasco, WA), Hooker, Brian S. (Kennewick, WA), Skeen, Rodney S. (Pendleton, OR)
10/ 214,655
August 7, 2002
[0002] The U.S. Government has a paid-up license in this invention and the right in limited circumstances to require the patent owner to license others on reasonable terms as provided for by the terms of contract number DE-AC0676RLO1830, awarded by the U.S. Department of Energy.