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PROCESS AND GENES FOR EXPRESSION ANDOVEREXPRESSION OF ACTIVE [FeFe] HYDROGENASES

United States Patent Application

*** PATENT GRANTED ***
20090087880
8,835,153
A1
View the Complete Application at the US Patent & Trademark Office
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A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.
Seibert, Michael (Lakewood, CO), King, Paul W. (Golden, CO), Ghirardi, Maria Lucia (Lakewood, CO), Posewitz, Matthew C. (Golden, CO), Smolinski, Sharon L. (Littleton, OH)
11/ 573,035
February 28, 2006
CONTRACTUAL ORIGIN OF THE INVENTION [0002] The United States Government has rights in this invention pursuant to Contract No. DE-AC36-99GO10337 between the United States Department of Energy and the Midwest Research Institute.