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Method and Apparatus for Bacterial Transformation by Electroporation with Waveforms Incorporating Pulsed Rf Between 3 and 125 Mhz

United States Patent Application

View the Complete Application at the US Patent & Trademark Office
A method and apparatus for electroporation includes placing a mixture of bacterial suspension and transforming DNA into an electroporation cuvette. The resulting sample is subjected through a current-limiting device to a complex 5 waveform including a burst of high-voltage radio-frequency current, which in some embodiments is superimposed on a biphasic high-voltage DC pulse, and in other embodiments on a high-voltage lower-frequency AC burst. The total waveform has at least an initial portion greater than eleven thousand volts per centimeter of electrode spacing, and a later portion in some embodiments is reduced to less than thirty percent 10 of magnitude of the initial portion. Transformed bacteria are selected by culture in selective medium in an embodiment. The high-voltage radio-frequency current is between 3 and 125 MHz, and in an embodiment is 24 MHz.
Tyurin, Mikhail V. (Lebanon, NH), Lynd, Lee R. (Meriden, NH), Sullivan, Charles R. (West Lebanon, NH)
11/ 579,640
May 6, 2005
US GOVERNMENT RIGHTS [0002] The United States Government has certain rights in this invention pursuant to contract Phase I SBIR DE-FG02-03ER83593 awarded by the Department of Energy.