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Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor

United States Patent Application

View the Complete Application at the US Patent & Trademark Office
National Renewable Energy Laboratory - Visit the NREL Technology Transfer Website
The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenic enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.
Maupin-Furlow, Julie A. (Gainesville, FL), Talarico, Lee Ann (Gainesville, FL), Raj, Krishnan Chandra (The Nilgiris, IN), Ingram, Lonnie O. (Gainesville, FL)
University of Florida Research Foundation, Inc. (Gainesville FL)
11/ 880,786
July 24, 2007
GOVERNMENT SPONSORED RESEARCH [0002] This work was supported, in part, by grants from the U.S. Department of Energy's National Renewable Energy Laboratory (ZDH-9-29009-04), Energy Biosciences Program (FG02-96ER20222), and the Florida Agricultural Experiment Station.