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Multiple displacement amplification with blocker DNA

United States Patent Application

View the Complete Application at the US Patent & Trademark Office
Lawrence Livermore National Laboratory - Visit the Industrial Partnerships Office Website
A method of whole genome amplification of a nucleic acid sample comprising providing a starting template of the nucleic acid sample, bringing the template together with blocking DNA, bringing the template together with DNA polymerase, and multiple displacement amplification of the template. The method can be used with a starting template of less than 1 ng of the nucleic acid sample.
Hara, Christine A. (Livermore, CA), Christian, Allen T. (Madison, WI)
The Regents of the University of California
11/ 509,996
August 24, 2006
MULTIPLE DISPLACEMENT AMPLIFICATION WITH BLOCKER DNA [0002] The United States Government has rights in this invention pursuant to Contract No. W-7405-ENG-48 between the United States Department of Energy and the University of California for the operation of Lawrence Livermore National Laboratory.