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Methods and compositions for the removal of nucleic acid amplification inhibitors

United States Patent Application

View the Complete Application at the US Patent & Trademark Office
Los Alamos National Laboratory - Visit the Technology Transfer Division Website
An improved method for preparing nucleic acid extracts from environmental samples contaminated by polymerase inhibitors such as humic and fulvic acids is provided. The methods of the invention utilize chemical compositions capable of precipitating humic and/or fulvic acids from organic samples. The methods may be used in connection with the preparation of DNA and RNA extracts, thereby providing purified preparations suitable for amplification using PCR, RT-PCR, and other nucleic acid amplification technologies. Reagent kits for preparing a purified nucleic acid-containing extract from an environmental sample of soil, fluid, or organic particles, using the chemical compositions of the invention are also provided.
Dunbar, John M. (Santa Fe, NM), Stark, Peter C. (Los Alamos, NM), Robison, Thomas W. (Los Alamos, NM), Lazarin, Raquel A. (Milwaukee, WI)
11/ 404,235
April 14, 2006
STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT [0002] This invention was made with government support under Contract No. W-7405-ENG-36 awarded by the United States Department of Energy to The Regents of The University of California. The government has certain rights in this invention.