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Nanogrid Rolling Circle DNA Sequencing

United States Patent Application

View the Complete Application at the US Patent & Trademark Office
The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.
Church, George M. (Brookline, MA), Porreca, Gregory J. (Cambridge, MA), Shendure, Jay (Seattle, WA), Rosenbaum, Abraham Meir (Brookline, MA)
15/ 452,777
March 8, 2017
STATEMENT OF GOVERNMENT INTERESTS [0002] This invention was made with government support under National Institutes of Health grant number HG003170 and under U.S. Department of Energy grant number DE-FG02-02ER63445. The Government has certain rights in the invention.