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Methods for Immortalization of Epithelial Cells

United States Patent Application

View the Complete Application at the US Patent & Trademark Office
Lawrence Berkeley National Laboratory - Visit the Technology Transfer and Intellectual Property Management Department Website
Methods for inducing non-clonal immortalization of normal epithelial cells by directly targeting the two main senescence barriers encountered by cultured epithelial cells. In finite lifespan pre-stasis human mammary epithelial cells (HMEC), the stress-associated stasis barrier was bypassed, and in post-stasis HMEC, the replicative senescence barrier, a consequence of critically shortened telomeres, was bypassed. Early passage non-clonal immortalized lines exhibited normal karyotypes. Methods of efficient HMEC immortalization, in the absence of "passenger" genomic errors, should facilitate examination of telomerase regulation and immortalization during human carcinoma progression, methods for screening for toxic and environmental effect on progression, and the development of therapeutics targeting the process of immortalization.
Stampfer, Martha R. (Oakland, CA), Garbe, James C. (San Francisco, CA), Vrba, Lukas (Tucson, AZ), Futscher, Bernard W. (Tucson, AZ)
The Regents of the University of California (Oakland CA), The Arizona Board of Regents on Behalf of the University of Arizona (Tucson AZ)
15/ 256,395
September 2, 2016
STATEMENT OF GOVERNMENTAL SUPPORT [0003] This invention was made with government support under Grant Nos. CA24844, AG033176, AG040081, CA23074 and CA65662 awarded by the National Institutes of Health, under Grant No. BCRP 060444 awarded by the Department of Defense, and under Contract No. DE-ACO2-05CH11231 awarded by the U.S. Department of Energy. The government has certain rights in the invention.