Skip to Content
Find More Like This
Return to Search

Multiplex RNA-Guided Genome Engineering

United States Patent Application

View the Complete Application at the US Patent & Trademark Office
Methods of multiplex genome engineering in cells using Cas9 is provided which includes a cycle of steps of introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to the target DNA and which guide the enzyme to the target DNA, wherein the one or more RNAs and the enzyme are members of a co-localization complex for the target DNA, and introducing into the cell a second foreign nucleic acid encoding one or more donor nucleic acid sequences, and wherein the cycle is repeated a desired number of times to multiplex DNA engineering in cells.
Church, George M. (Brookline, MA), Dicarlo, James (Boston, MA)
14/ 903,719
July 8, 2014
STATEMENT OF GOVERNMENT INTERESTS [0002] This invention was made with government support under DE-FG02-02ER63445 from the Department of Energy, NSF-SynBERC from the National Science Foundation and SA5283-11210 from the National Science Foundation. The government has certain rights in the invention.