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Rab9 protein crystal structures and methods for identifying Rab9 modulators

United States Patent Application

View the Complete Application at the US Patent & Trademark Office
Argonne National Laboratory - Visit the Technology Development and Commercialization Website
The present invention relates to crystalline Rab9 in complex with either GDP or the GTP analog Guanosine 5'-(.beta.,.gamma.-imido) triphosphate (GppNHp), the three dimensional coordinates and structures of Rab9 in Rab9-GDP and Rab9-GppNHp complexes, and uses thereof for drug design. In particular, the present invention relates to methods for producing Rab9 crystals of sufficient quality to obtain a determination of the three dimensional structure of Rab9 to a high resolution in both its GTP-bound (on/active) and GDP-bound (off/inactive) conformations. The present invention also relates to a computer-readable medium encoded with the three dimensional coordinates of Rab9 in Rab9-GDP and Rab9-GppNHp complexes wherein, using a graphical display software program, the three dimensional coordinates create an electronic file that can be visualized on a computer capable of representing the electronic file as a three dimensional image.
Meehan, Edward J. (Huntsville, AL), Chen, Liqing (Huntsville, AL), DiGiammarino, Enrico L. (Woodstock, GA), Hodge, Thomas W. (Athens, GA)
The University of Alamaba in Huntsville (Huntsville AL)
11/ 154,203
June 16, 2005
FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT [0002] The research underlying this invention was supported in part with funds from the National Science Foundation (NSF; NSF EPSCoR Program Grant No. EPS-0091853), the National Aeronautics and Space Administration (NASA; NASA Cooperative Agreement Number NCC8-243), and the United States Department of Energy (DOE; Contract No. W-31-109-Eng-38). The United States Government may have an interest in the subject matter of this invention.