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Compositions and methods for selecting open reading frames

United States Patent Application

View the Complete Application at the US Patent & Trademark Office
The current invention provides method and vectors for selecting open reading frames. Open reading frames present in a fragment of DNA cloned into the vectors of the invention result in creation of a fusion protein between the amino acid sequence encoded by the fusion protein and a reporter protein. The vector further comprises recombination sites so that once a recombinant that comprises an open reading frame is identified, either the reporter sequence or the open reading frame can be removed from the vector.
Bradbury, Andrew R.M. (Santa Fe, NM), Marzari, Roberto (Trieste, IT)
Los Alamos National Laboratory (Los Alamos NM)
10/ 175,689
June 19, 2002
[0001] This invention was made with government support under grant number DE-FG02-98ER62647 awarded by the Department of Energy. The Government has certain rights in this invention.