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Carbon NanoFiber Systems for Tissue Interfacing

Platform Technology for Electrophysiological Interfacing and for Drug and Gene Delivery

Oak Ridge National Laboratory

Contact ORNL About This Technology


Technology Marketing Summary

Scientists at ORNL have created vertically aligned carbon nano?fibers (VACNF) that are well suited for cell and tissue interfacing applications, such as electrophysiological stimulus and recording, and drug and gene delivery. VACNFs are produced in an uniform manner that allows for structural controls over the location, length, diameter, conicity, and surface composition.
Preliminary data with nanoelectrode arrays show effective tissue stimulation due to improved contact with tissue and higher current density. VACNFs also provide cell delivery with the advantage of a scalable nano?array that can address a multitude of cells. This technology can also be incorporate in microinjection?based therapies in which the VACNF is tethered with DNA providing a mechanism to control gene fate and new approaches for gene and cell therapy


 

Description

Scientists at ORNL have created vertically aligned carbon nano?fibers (VACNF) that are well suited for cell and tissue interfacing applications, such as electrophysiological stimulus and recording, and drug and gene delivery. VACNFs are produced in an uniform manner that allows for structural controls over the location, length, diameter, conicity, and surface composition.

 

Benefits
  • A variety of materials such as micro? and nano?electrode arrays, flexible sheets of the carbon nanofiber arrays, and drug and DNA delivery microarrays
  • Multiuse stimulus and electrophysiological monitoring in organotypic tissue culture, with high spatial resolutions
  • Measurements of both membrane depolarization and cell signaling species in cultures, such as dissociated neuronal cells
  • Modified VACNFs provide analyte sensitivity for electroanalytical applications
  • Material for intracellular drug and gene delivery
Applications and Industries

Preliminary data with nanoelectrode arrays show effective tissue stimulation due to improved contact with tissue and higher current density. VACNFs also provide cell delivery with the advantage of a scalable nano?array that can address a multitude of cells. This technology can also be incorporate in microinjection?based therapies in which the VACNF is tethered with DNA providing a mechanism to control gene fate and new approaches for gene and cell therapy.

More Information

Patents:
Individually electrically addressable vertically aligned carbon nanofibers on insulating substrates (UTB – ID 978) , US Patent 6,982,519
Individually electrically addressable carbon nanofibers on insulating substrates (UTB – ID 978), US Patent 7,144,287
Parallel Macromolecular Delivery and Biochemical/Electrochemical Interface to Cells Employing Nanostructures (UTB – ID 1199), US Patent Application 10/408,294
Method and Apparatus for Sustaining Viability of Biological Cells on a Substrate (UTB – ID 1482 and UTB – ID 1483), US Patent Application US08/051190

Lead Inventor: Tim McKnight
 

Patents and Patent Applications
ID Number
Title and Abstract
Primary Lab
Date
Patent 6,982,519
Patent
6,982,519
Individually electrically addressable vertically aligned carbon nanofibers on insulating substrates
Systems and methods are described for individually electrically addressable carbon nanofibers on insulating substrates. An apparatus includes an electrically conductive interconnect formed on at least a part of an insulating surface on a substrate; and at least one vertically aligned carbon nanofiber coupled to the electrically conductive interconnect. A kit includes a substrate having an insulating surface; an electrically conductive interconnect formed on at least a part of the insulating surface; and at least one vertically aligned carbon nanofiber coupled to the electrically conductive interconnect.
01/03/2006
Issued
Patent 7,144,287
Patent
7,144,287
Individually electrically addressable carbon nanofibers on insulating substrates
Systems and methods are described for individually electrically addressable carbon nanofibers on insulating substrates. A method includes forming an electrically conductive interconnect on at least a part of an insulating surface on a substrate; and growing at least one fiber that is coupled to the electrically conductive interconnect.
12/05/2006
Issued
Patent 8,076,124
Patent
8,076,124
Method and apparatus for sustaining viability of biological cells on a substrate
A method for the transient transformation of a living biological cell having an intact cell membrane defining an intracellular domain, and an apparatus for the transient transformation of biological cells. The method and apparatus include introducing a compartmentalized extracellular component fixedly attached to a cellular penetrant structure to the intracellular domain of the cell, wherein the cell is fixed in a predetermined location and wherein the component is expressed within in the cell while being retained within the compartment and wherein the compartment restricts the mobility and interactions of the component within the cell and prevents transference of the component to the cell.
Oak Ridge National Laboratory 12/13/2011
Issued
Patent 8,993,327
Patent
8,993,327
Parallel macromolecular delivery and biochemical/electrochemical interface to cells employing nanostructures
Systems and methods are described for parallel macromolecular delivery and biochemical/electrochemical interface to whole cells employing carbon nanostructures including nanofibers and nanotubes. A method includes providing a first material on at least a first portion of a first surface of a first tip of a first elongated carbon nanostructure; providing a second material on at least a second portion of a second surface of a second tip of a second elongated carbon nanostructure, the second elongated carbon nanostructure coupled to, and substantially parallel to, the first elongated carbon nanostructure; and penetrating a boundary of a biological sample with at least one member selected from the group consisting of the first tip and the second tip.
Oak Ridge National Laboratory 03/31/2015
Issued
Technology Status
Technology IDDevelopment StageAvailabilityPublishedLast Updated
978, 1199, 1482, 1483DevelopmentAvailable09/21/201209/21/2012

Contact ORNL About This Technology

To: Jennifer Tonzello Caldwell, Ph.D.<pftt@ornl.gov>