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Low-cost, Rapid DNA Sequencing Technique

Oak Ridge National Laboratory

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Publications:

PDF Document Publication11-G00264_ID2098.pdf (476 KB)

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<p><span style="font-size: x-small;">Schematic model of setup of nano-gap between two electrodes, with (a) and without (b) a molecule between them.</span></p>

 

Schematic model of setup of nano-gap between two electrodes, with (a) and without (b) a molecule between them.

Technology Marketing SummaryA faster, less expensive, more accurate and more reliable method for DNA sequencing has been developed at ORNL. The method takes a high-frequency measurement on a single strand of DNA or RNA deposited on a substrate using an alternating current (AC). The data is used as a criterion to identify the genetic sequence.DescriptionSequencing DNA is crucial for future breakthroughs in biological and biomedical research. Until now, DNA sequencing for medical applications has been restricted by the high cost of the work. The technology includes one electrode located on the dielectric surface of a substrate and a second that is separated from the first by a gap enabling a nucleic acid strand to pass through. An AC capacitance measurement assembly is connected to the first and second electrodes and configured to generate measurement data. The nucleic acid strand transport mechanism located on the dielectric surface of the substrate is also configured to generate measurement data.Benefits•Low-cost and rapid DNA sequencing
•Increased accuracy and reliability
•Could be combined with ORNL’s Nanoscopic Electrode Molecular Probes, US Patent Application 12/321,823 if nanoscale electrodes for DNA sequencing is required
Applications and Industries•Medical and pharmaceutical industries
•Basic science research
More InformationXiaoguang Zhang and Jun-qiang Lu, Nucleotide Capacitance Measurement for Low Cost DNA Sequencing, U.S. Patent Application 12/848,407, filed August 2, 2010.Patents and Patent Applications
ID Number
Title and Abstract
Primary Lab
Date
Application 20110037486
Application
20110037486
NUCLEOTIDE CAPACITANCE MEASUREMENT FOR LOW COST DNA SEQUENCING
High frequency capacitance measurement on a single strand of deoxyribonucleic acid (DNA) or a ribonucleic acid (RNA) is employed to provide identification of the nucleotides in the strand. Effect of variations in the capacitance of nucleotides can be minimized by employing statistical quantities generated from multiple measurement values on a strand of DNA or RNA nucleotides, or by employing a program that positively identifies a large capacitance nucleotide upon detection of a large capacitance. Capacitance data on a DNA strand can be used as a criterion for identifying the DNA sequence in conjunction with other methods for identifying the DNA sequence.
Oak Ridge National Laboratory 08/02/2010
Filed
Patent 8,183,648
Patent
8,183,648
Nanoscopic electrode molecular probes
The present invention relates to a method and apparatus for enhancing the electron transport property measurements of a molecule when the molecule is placed between chemically functionalized carbon-based nanoscopic electrodes to which a suitable voltage bias is applied. The invention includes selecting a dopant atom for the nanoscopic electrodes, the dopant atoms being chemically similar to atoms present in the molecule, and functionalizing the outer surface and terminations of the electrodes with the dopant atoms.
Oak Ridge National Laboratory 05/22/2012
Issued
Technology Status
Technology IDDevelopment StageAvailabilityPublishedLast Updated
UT-B ID 200802098ProposedAvailable11/21/201110/27/2011

Contact ORNL About This Technology

To: David L. Sims<simsdl@ornl.gov>