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Universal Gene Transfer Technology for Gram Positive Bacteria

Oak Ridge National Laboratory

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PDF Document Publication11-G00255_ID2139_rev.pdf (493 KB)


Technology Marketing SummaryA genetic engineering technology invented at ORNL facilitates DNA delivery to a cell by using ultrasound to permeate the cell’s plasma membrane. DNA delivery using this technology is simple, quick, inexpensive, and offers a universal method for gene transfer.DescriptionExisting methods for DNA delivery all have significant drawbacks, including causing significant damage to the membrane of a cell. These conventional methods require repeated rounds of washing and other treatments, prior to DNA transformation, making the protocol complex and difficult. The ORNL invention provides a sonoporation-based method that using ultrasonic frequencies to effectively modify the permeability of the cell plasma membrane prior to inserting a chosen compound.BenefitsThe method can be universally applied to deliver nucleic acids, proteins, lipids, carbohydrates, viruses, small organic and inorganic molecules, and nanoparticles to Gram positive bacteria, including Bacillus, Streptococcus, Acetobacterium, and Clostridium.
•Greatly simplified means of genetic manipulation using sonoporation
•Straightforward, quick, easy, and costeffective process
•Due to being a mechanical method, the invention may be well suited for many different cell types and tissues
Applications and Industries•Medical, plant biology, and biopharmaceutical research
•Gene therapy holds great promise to cure cancer, HIV, and other hereditary and contagious diseases
•Genetic engineering has been widely adopted in plant science to deliver economical crops
•Transgenic bacteria or fungi in microbiology can be manipulated to produce cheap biopharmaceuticals
More InformationYunfeng Yang and Youngchao Li, Transformation of Gram Positive Bacteria by Sonoporation, U.S. Patent Application 20100196983, filed January 29, 2010.Patents and Patent Applications
ID Number
Title and Abstract
Primary Lab
Date
Patent 8,669,085
Patent
8,669,085
Transformation of gram positive bacteria by sonoporation
The present invention provides a sonoporation-based method that can be universally applied for delivery of compounds into Gram positive bacteria. Gram positive bacteria which can be transformed by sonoporation include, for example, Bacillus, Streptococcus, Acetobacterium, and Clostridium. Compounds which can be delivered into Gram positive bacteria via sonoporation include nucleic acids (DNA or RNA), proteins, lipids, carbohydrates, viruses, small organic and inorganic molecules, and nano-particles.
Oak Ridge National Laboratory 03/11/2014
Issued
Technology Status
Technology IDDevelopment StageAvailabilityPublishedLast Updated
UT-B ID 200802139ProposedAvailable11/21/201110/27/2011

Contact ORNL About This Technology

To: Nestor Fronco<francone@ornl.gov>