Skip to Content
Find More Like This
Return to Search

Mutant Fatty Acid Desaturase and Method for Directed Mutagenesis

Brookhaven National Laboratory

Contact BNL About This Technology

Technology Marketing Summary

This technology provides methods for specifically optimizing the activity of enzynes in a desired direction. By identifying the amino acids in the active site of the enzymes that participate in catalytic activity, this method provides a way to simultaneously select the optimal amino acid to replace each native residue such that mutant enzymes with optimally charged activities are discovered.

Description

The method for site-directed mutagenesis is specifically applied to fatty acid desaturase enzymes to derive enzymes that have significantly higher enzymatic activity towards substrates with fewer than 18 carbon atom chains. (Continuation in part of patent 6,686,186).

Benefits

The method can be used to alter the function of a protein by producing a library of mutants of the protein using site directed mutagenesis and selecting for mutants which exhibit the desired alteration of function. Although this method is applicable to any enzyme for which a selection method is available, it has been specifically applied to acyl ACP desaturases.

Applications and Industries

The cDNA fragments encoding these mutant desaturases can be introduced via expression vectors into prokaryotic and eukaryotic cells and can be used in the production of transgenic plants. These mutant forms of the fatty acid desaturase enzyme offer a way to modify the fatty acid content and composition of oilseed crops.

Patents and Patent Applications
ID Number
Title and Abstract
Primary Lab
Date
Patent 7,323,335
Patent
7,323,335
Mutant fatty acid desaturase and methods for directed mutagenesis
The present invention relates to methods for producing fatty acid desaturase mutants having a substantially increased activity towards substrates with fewer than 18 carbon atom chains relative to an unmutagenized precursor desaturase having an 18 carbon chain length specificity, the sequences encoding the desaturases and to the desaturases that are produced by the methods. The present invention further relates to a method for altering a function of a protein, including a fatty acid desaturase, through directed mutagenesis involving identifying candidate amino acid residues, producing a library of mutants of the protein by simultaneously randomizing all amino acid candidates, and selecting for mutants which exhibit the desired alteration of function. Candidate amino acids are identified by a combination of methods. Enzymatic, binding, structural and other functions of proteins can be altered by the method.
Brookhaven National Laboratory 01/29/2008
Issued
Technology Status
Technology IDDevelopment StageAvailabilityPublishedLast Updated
BSA 04-11PrototypeAvailable03/30/201503/30/2015

Contact BNL About This Technology

To: Poornima Upadhya<pupadhya@bnl.gov>