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Cloning and expression of the gene for bacteriophage T7 RNA polymerase

United States Patent

5,869,320
February 9, 1999
View the Complete Patent at the US Patent & Trademark Office
Brookhaven National Laboratory - Visit the Office of Technology Commercialization and Partnerships Website
This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the R7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.
Studier; F. William (Stony Brook, NY), Davanloo; Parichehre (Basel, CH), Rosenberg; Alan H. (Setauket, NY), Moffatt; Barbara A. (Waterloo, CA), Dunn; John J. (Bellport, NY)
Brookhaven Science Associates LLC (Upton, NY)
08/ 784,201
January 15, 1997
This invention was made with Government support under contract number DE-AC02-76CH00016, between the U.S. Department of Energy and Associated Universities, Inc. The Government has certain rights in the invention.