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Cloning and developmental expression of pea ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit epsilon N-methyltransferase

United States Patent

5,866,394
February 2, 1999
View the Complete Patent at the US Patent & Trademark Office
The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .sup..epsilon. N-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the .epsilon.-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant are also disclosed.
Houtz; Robert L. (Lexington, KY)
University of Kentucky Research Foundation (Lexington, KY)
08/ 741,931
October 31, 1996
IDENTIFICATION OF FEDERAL FUNDING The present invention was supported by U.S. Department of Energy Grant DE-FG05-92ER20075.