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Method for construction of normalized cDNA libraries

United States Patent

November 3, 1998
View the Complete Patent at the US Patent & Trademark Office
This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries.
Soares; Marcelo B. (New York, NY), Efstratiadis; Argiris (Englewood, NJ)
The Trustees of Columbia University in the City of New York (New York, NY)
08/ 619,542
June 21, 1996
This invention was made with support under Grant Number DE-FG0291ER61233 from the U.S. Department of Energy. Accordingly, the U.S. Government has certain rights in the invention.