Skip to Content
Find More Like This
Return to Search

Method for construction of normalized cDNA libraries

United States Patent

5,830,662
November 3, 1998
View the Complete Patent at the US Patent & Trademark Office
This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries.
Soares; Marcelo B. (New York, NY), Efstratiadis; Argiris (Englewood, NJ)
The Trustees of Columbia University in the City of New York (New York, NY)
08/ 619,542
June 21, 1996
This invention was made with support under Grant Number DE-FG0291ER61233 from the U.S. Department of Energy. Accordingly, the U.S. Government has certain rights in the invention.