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Sequence independent amplification of DNA

United States Patent

5,731,171
March 24, 1998
View the Complete Patent at the US Patent & Trademark Office
The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.
Bohlander; Stefan K. (Chicago, IL)
Arch Development Corp. (Chicago, IL)
08/ 096,637
July 23, 1993
The government owns rights in the present invention pursuant to grants numbers CA49133, CA42557 and CA40046 from the National Institutes of Health and to grant number DE-FG02-86ER60408 from the Department of Energy .