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Method for increasing thermostability in cellulase ennzymes

United States Patent

5,712,142
January 27, 1998
View the Complete Patent at the US Patent & Trademark Office
National Renewable Energy Laboratory - Visit the NREL Technology Transfer Website
Cellulase Enzymes for the Conversion of Biomass to Biofuels and Chemicals
The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in Pichia pastoris. A new modified E1 endoglucanase enzyme comprising the catalytic domain of the full size E1 enzyme demonstrates enhanced thermostability and is produced by two methods. The first method of producing the new modified E1 is proteolytic cleavage to remove the cellulose binding domain and linker peptide of the full size E1. The second method of producing the new modified E1 is genetic truncation of the gene encoding the full size E1 so that the catalytic domain is expressed in the expression product.
Adney; William S. (Golden, CO), Thomas; Steven R. (Denver, CO), Baker; John O. (Golden, CO), Himmel; Michael E. (Littleton, CO), Chou; Yat-Chen (Wheat Ridge, CO)
Midwest Research Institute (Kansas City, MO)
08/ 604,913
February 22, 1996
The United States Government has rights in this invention under Contract No. DE-AC36-83CH10093 between the United States Department of Energy and the National Renewable Energy Laboratory, a Division of the Midwest Research Institute.