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Pentose fermentation by recombinant zymomonas

United States Patent

5,712,133
January 27, 1998
View the Complete Patent at the US Patent & Trademark Office
National Renewable Energy Laboratory - Visit the NREL Technology Transfer Website
The invention relates to microorganisms which normally do not ferment pentose sugar and which are genetically altered to ferment pentose sugar to produce ethanol, and fermentation processes utilizing the same. Examples include Zymomonas mobilis which has been transformed with combinations of E. coli genes for xylose isomerase, xylulokinase, transaldolase, transketolase, L-arabinose isomerase, L-ribulokinase, and L-ribulose 5-phosphate 4-epimerase. Expression of the added genes are under the control of Zymomonas mobilis promoters. These newly created microorganisms are useful for fermenting pentoses and glucose, produced by hydrolysis of hemicellulose and cellulose, to produce ethanol.
Picataggio; Stephen K. (Golden, CO), Zhang; Min (Lakewood, CO), Eddy; Christina K. (Saratoga Springs, NY), Deanda; Kristine A. (Conifer, CO), Finkelstein; Mark (Fort Collins, CO), Mohagheghi; Ali (Northglenn, CO), Newman; Mildred M. (Littleton, CO), McMillan; James D. (Boulder, CO)
Midwest Research Institute (Kansas City, MO)
08/ 422,424
April 14, 1995
CONTRACTUAL ORIGIN OF THE INVENTION The United States Government has rights in this invention pursuant to Contract No. DE-AC36-83CH10093 between the United States Department of Energy and the Midwest Research Institute.