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Procedure for normalization of cDNA libraries

United States Patent

5,702,898
December 30, 1997
View the Complete Patent at the US Patent & Trademark Office
This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library.
Bonaldo; Maria DeFatima (New York, NY), Soares; Marcelo Bento (New York, NY)
The Trustees of Columbia University in The City of New York (New York, NY)
08/ 465,857
June 6, 1995
The invention disclosed herein was made with Government support under Grant No. R01-HG00980 from the National Institutes of Health, U.S. Department of Health and Human Services and Grant No. DE-FG02-91ER61233 from the U.S. Department of Energy. Accordingly, the U.S. Government has certain rights in this invention.