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Method and apparatus for accurately manipulating an object during microelectrophoresis

United States Patent

*** EXPIRED ***
September 23, 1997
View the Complete Patent at the US Patent & Trademark Office
Lawrence Berkeley National Laboratory - Visit the Technology Transfer and Intellectual Property Management Department Website
An apparatus using electrophoresis provides accurate manipulation of an object on a microscope stage for further manipulations add reactions. The present invention also provides an inexpensive and easily accessible means to move an object without damage to the object. A plurality of electrodes are coupled to the stage in an array whereby the electrode array allows for distinct manipulations of the electric field for accurate manipulations of the object. There is an electrode array control coupled to the plurality of electrodes for manipulating the electric field. In an alternative embodiment, a chamber is provided on the stage to hold the object. The plurality of electrodes are positioned in the chamber, and the chamber is filled with fluid. The system can be automated using visual servoing, which manipulates the control parameters, i.e., x, y stage, applying the field, etc., after extracting the significant features directly from image data. Visual servoing includes an imaging device and computer system to determine the location of the object. A second stage having a plurality of tubes positioned on top of the second stage, can be accurately positioned by visual servoing so that one end of one of the plurality of tubes surrounds at least part of the object on the first stage.
Parvin; Bahram A. (Hercules, CA), Maestre; Marcos F. (Berkeley, CA), Fish; Richard H. (Berkeley, CA), Johnston; William E. (Kensington, CA)
The Regents, University of California (Oakland, CA)
08/ 423,969
April 18, 1995
ORIGIN OF THE INVENTION This invention was made with Government support under Contract No. DE-AC03-76SF00098 awarded by the U.S. Department of Energy, Office of Scientific Computing, under Grant No. AIO8427-25 awarded by the National Institute of Health and under a Laboratory Directed Research and Development grant awarded by the Director of Lawrence Berkeley Laboratory. The Government has certain rights in this invention.