The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed.
This invention was made with Government support under Grant nos. DE-FG02-89ER-60862 and DE-FG02-90ER-610 by the Department of Energy. The Government has certain rights in the invention.