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Transposon-containing DNA cloning vector and uses thereof

United States Patent

5,645,991
July 8, 1997
View the Complete Patent at the US Patent & Trademark Office
The present invention discloses a rapid method of restriction mapping, sequencing or localizing genetic features in a segment of deoxyribonucleic acid (DNA) that is up to 42 kb in size. The method in part comprises cloning of the DNA segment in a specialized cloning vector and then isolating nested deletions in either direction in vivo by intramolecular transposition into the cloned DNA. A plasmid has been prepared and disclosed.
Berg; Claire M. (W. Willington, CT), Berg; Douglas E. (St. Louis, MO), Wang; Gan (Storrs, CT)
Univ. of Connecticut (Storrs, CT), Washington University (St. Louis, MO)
08/ 403,582
March 14, 1995
GOVERNMENT SUPPORT This invention was made with Government support under Grant nos. DE-FG02-89ER-60862 and DE-FG02-90ER-610 by the Department of Energy. The Government has certain rights in the invention.