Recombinant organisms are disclosed that contain a pathway for glucose uptake other than the pathway normally utilized by the host cell. In particular, the host cell is one in which glucose transport into the cell normally is coupled to PEP production. This host cell is transformed so that it uses an alternative pathway for glucose transport that is not coupled to PEP production. In a preferred embodiment, the host cell is a bacterium other than Z. mobilis that has been transformed to contain the glf and glk genes of Z. mobilis. By uncoupling glucose transport into the cell from PEP utilization, more PEP is produced for synthesis of products of commercial importance from a given quantity of biomass supplied to the host cells.
The present invention was made with government support provided, through the Florida Agricultural Experiment Station, under USDA Alcohol Fuels Program grants Nos. 86CRCR12134, 88-37233-3987 and under DOE Office of Basic Energy grant FG05-86ER13574. The federal government has certain rights in the present invention.