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Rapid purification of circular DNA by triplex-mediated affinity capture

United States Patent

5,591,841
January 7, 1997
View the Complete Patent at the US Patent & Trademark Office
A single-step capture of a target supercoiled double-stranded DNA molecule is accomplished by forming a local triple-helix among two strands of the supercoiled circular DNA and an oligonucleotide probe. The oligonucleotide is bound to an immobilizing support which facilitates the immobilization and purification of target DNA molecules. Non-target DNA molecules and other contaminating cellular material are easily removed by washing. The triple-helical structure is destabilized by raising the pH, leaving purified target DNA in the supernatant and reusable affinity capture oligonucleotide secured to the immobilizing support.
Ji; Huamin (Madison, WI), Smith; Lloyd M. (Madison, WI)
08/ 317,102
September 30, 1994
GOVERNMENT RIGHTS This invention was made with government support under Contract Number DE-FG02-90ER61026 awarded by the U.S. Department of Energy. The government has certain rights in this invention.