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Multiple capillary biochemical analyzer with barrier member

United States Patent

*** EXPIRED ***
5,567,294
October 22, 1996
View the Complete Patent at the US Patent & Trademark Office
A multiple capillary biochemical analyzer for sequencing DNA and performing other analyses, in which a set of capillaries extends from wells in a microtiter plate into a cuvette. In the cuvette the capillaries are held on fixed closely spaced centers by passing through a sandwich construction having a pair of metal shims which squeeze between them a rubber gasket, forming a leak proof seal for an interior chamber in which the capillary ends are positioned. Sheath fluid enters the chamber and entrains filament sample streams from the capillaries. The filament sample streams, and sheath fluid, flow through aligned holes in a barrier member spaced close to the capillary ends, into a collection chamber having a lower glass window. The filament streams are illuminated above the barrier member by a laser, causing them to fluoresce. The fluorescence is viewed end-on by a CCD camera chip located below the glass window. The arrangement ensures an equal optical path length from all fluorescing spots to the CCD chip and also blocks scattered fluorescence illumination, providing more uniform results and an improved signal to noise ratio.
Dovichi; Norman J. (Edmonton, CA), Zhang; Jian Z. (Edmonton, CA)
Board of Governors, University of Alberta (Alberta, CA)
08/ 593,877
January 30, 1996
FEDERAL RIGHTS IN THE INVENTION The U.S. government has rights in this invention pursuant to grant number DE-FG02-92ER61123 awarded by the U.S. Department of Energy.