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Low molecular weight thermostable .beta.-D-glucosidase from acidothermus cellulolyticus

United States Patent

*** EXPIRED ***
5,432,075
July 11, 1995
View the Complete Patent at the US Patent & Trademark Office
National Renewable Energy Laboratory - Visit the NREL Technology Transfer Website
Cellulase Enzymes for the Conversion of Biomass to Biofuels and Chemicals
A purified low molecular weight .beta.-D-glucosidase is produced from Acidothermus cellulolyticus ATCC 43068. The enzyme is water soluble, possesses activity against pNP-.beta.-D-glucopyranoside, has a high of degree of stability toward heat, exhibits optimal temperature activity at about 65.degree. C. at a pH range of from about 2 to about 7, has an inactivation temperature of about 80.degree. C. at a pH range of from about 2 to about 7 and has a molecular weight of about 50.5-54.5 kD as determineded by SDS-PAGE.
Himmel; Michael E. (Littleton, CO), Tucker; Melvin P. (Lakewood, CO), Adney; William S. (Golden, CO), Nieves; Rafael A. (Lakewood, CO)
Midwest Research Institute (Kansas City, MO)
08/ 275,995
July 15, 1994
The United States Government has rights in this invention pursuant to Contract No. DE-AC. 36-83CH10093 between the United States Department of Energy and the Midwest Research Institute.