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Method for delivery of small molecules and proteins across the cell wall of algae using molecular transporters

United States Patent

9,816,066
November 14, 2017
View the Complete Patent at the US Patent & Trademark Office
Lawrence Berkeley National Laboratory - Visit the Technology Transfer and Intellectual Property Management Department Website
The introduction of tools to study, control or expand the inner-workings of algae has been slow to develop. Provided are embodiments of a molecular method based on guanidinium-rich molecular transporters (GR-MoTrs) for bringing molecular cargos into algal cells. The methods of the disclosure have been shown to work in wild-type algae that have an intact cell wall. Developed using Chlamydomonas reinhardtii, this method is also successful with less studied algae, including Neochloris oleoabundans and Scenedesmus dimorphus, thus providing a new and versatile tool for algal research and modification. The method of delivering a cargo compound to an algal cell comprises contacting an algal cell with a guanidinium-rich delivery vehicle comprising a guanidinium-rich molecular transporter (GR-MoTr) linked to a cargo compound desired to be delivered to the algal cell, whereby the guanidinium-rich molecular transporter can traverse the algal cell wall, thereby delivering the cargo compound to the algal cell.
Geihe; Erika (San Franciso, CA), Trantow; Brian (Menlo Park, CA), Wender; Paul (Menlo Park, CA), Hyman; Joel M. (San Mateo, CA), Parvin; Bahram (Mill Valley, CA)
The Regents of the University of California (Oakland, CA), The Board of Trustees of the Leland Stanford Junior University (Palo Alto, CA)
14/ 396,274
20150118704
March 12, 2013
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT This invention was made with Government support under contract DE-ACO2-05CH11231 awarded by the Department of Energy, under contract CA31841 awarded by the National Institutes of Health, and under contract CA31845 awarded by the National Institutes of Health. The Government has certain rights in this invention.