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Method and apparatus for purifying nucleic acids and performing polymerase chain reaction assays using an immiscible fluid

United States Patent

9,803,238
October 31, 2017
View the Complete Patent at the US Patent & Trademark Office
Sandia National Laboratories - Visit the Intellectual Property Management and Licensing Website
Embodiments of the present invention are directed toward devices, systems, and methods for purifying nucleic acids to conduct polymerase chain reaction (PCR) assays. In one example, a method includes generating complexes of silica beads and nucleic acids in a lysis buffer, transporting the complexes through an immiscible fluid to remove interfering compounds from the complexes, further transporting the complexes into a density medium containing components required for PCR where the nucleic acids disassociate from the silica beads, and thermocycling the contents of the density medium to achieve PCR. Signal may be detected from labeling agents in the components required for PCR.
Koh; Chung-Yan (Dublin, CA), Light; Yooli Kim (Pleasanton, CA), Piccini; Matthew Ernest (Belmont, CA), Singh; Anup K. (Danville, CA)
National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)
14/ 090,040
November 26, 2013
STATEMENT REGARDING RESEARCH & DEVELOPMENT This invention was developed under Contract DE-AC04-94AL85000 between Sandia Corporation and the U.S. Department of Energy. The U.S. Government has certain rights in the invention.