Skip to Content
Find More Like This
Return to Search

Nanogrid rolling circle DNA sequencing

United States Patent

April 18, 2017
View the Complete Patent at the US Patent & Trademark Office
The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.
Church; George M. (Brookline, MA), Porreca; Gregory J. (Ocean City, NJ), Shendure; Jay (Chagrin Fall, OH), Rosenbaum; Abraham Meir (Brookline, MA)
President and Fellows of Harvard College (Cambridge, MA)
12/ 120,541
May 14, 2008
STATEMENT OF GOVERNMENT INTERESTS This invention was made with government support under National Institutes of Health grant number HG003170 and under U.S. Department of Energy grant number DE-FG02-02ER63445. The Government has certain rights in the invention.