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Intein-modified enzymes, their production and industrial applications

United States Patent

October 11, 2016
View the Complete Patent at the US Patent & Trademark Office
A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-.beta.-sheet junction or a loop-.alpha.-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.
Apgar; James (Somerville, MA), Lessard; Philip (Framingham, MA), Raab; R. Michael (Arlington, MA), Shen; Binzhang (Jamaica, MA), Lazar; Gabor (Belmont, MA), de la Vega; Humberto (Peabody, MA)
Agrivida, Inc. (Medford, MA)
13/ 508,234
November 5, 2010
This invention was made at least in part with government support under the United States Department of Energy Advanced Research Projects Agency-Energy (ARPA-e) Grant No. DE-AR0000042. The Government has certain rights in this invention.