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Increasing cell culture population doublings for long-term growth of finite life span human cell cultures

United States Patent

June 28, 2016
View the Complete Patent at the US Patent & Trademark Office
Lawrence Berkeley National Laboratory - Visit the Technology Transfer and Intellectual Property Management Department Website
Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.
Stampfer; Martha R. (Oakland, CA), Garbe; James C. (San Francisco, CA)
The Regents of the University of California (Oakland, CA)
14/ 627,888
February 20, 2015
STATEMENT OF GOVERNMENTAL SUPPORT This invention was made during work supported by NIH grant CA-24844, NIH grant U54 CA112970-01 and the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The government has certain rights in this invention.