Genetically-engineered cyanochrome fluorophore molecules (fluorophores) with increased fluorescence and with absorbing fluorescence in the blue and green (blue/green) portion of the light spectrum are provided. These fluorophores are derived from the domains of phytochromes, and in particular cyanobacterial phytochromes. Methods for generating these fluorophores and various applications of these fluorophores are also provided.
This invention was made with government support under DE-FG02-88ER13968 awarded by the US Department of Energy and 0719153 awarded by the National Science Foundation. The government has certain rights in the invention.