Methods of lipid extraction from biomass, in particular wet algae, through conventionally heated subcritical water, and microwave-assisted subcritical water. In one embodiment, fatty acid methyl esters from solids in a polar phase are further extracted to increase biofuel production.
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
This invention was made with government support under Contracts No. DE-EE0003046, FA8650-11-C-2127, awarded by the US Department of Energy, US Air Force Research Laboratory respectively, and Contracts No. EEC-1028968, MRI DBI-0520956 awarded by the National Science Foundation.