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Methods for determining enzymatic activity comprising heating and agitation of closed volumes

United States Patent

March 15, 2016
View the Complete Patent at the US Patent & Trademark Office
Idaho National Laboratory - Visit the Technology Transfer and Commercialization Office Website
Methods for determining thermophilic enzymatic activity include heating a substrate solution in a plurality of closed volumes to a predetermined reaction temperature. Without opening the closed volumes, at least one enzyme is added, substantially simultaneously, to the closed volumes. At the predetermined reaction temperature, the closed volumes are agitated and then the activity of the at least one enzyme is determined. The methods are conducive for characterizing enzymes of high-temperature reactions, with insoluble substrates, with substrates and enzymes that do not readily intermix, and with low volumes of substrate and enzyme. Systems for characterizing the enzymes are also disclosed.
Thompson; David Neil (Idaho Falls, ID), Henriksen; Emily DeCrescenzo (Morrisville, NC), Reed; David William (Idaho Falls, ID), Jensen; Jill Renee (Buffalo Grove, IL)
Battelle Energy Alliance, LLC (Idaho Falls, ID)
14/ 180,161
February 13, 2014
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT This invention was made with government support under Contract No. DE-AC07-05-ID14517 awarded by the United States Department of Energy. The government has certain rights in the invention.